Intracytoplasmic sperm injection or ICSI - often referred to as micromanipulation - is a revolutionary assisted reproduction technique used to treat male factor infertility. ... The procedure starts with retrieving eggs from the woman, then injecting a single sperm directly into each egg to achieve fertilization.ICSI is the most successful form of treatment for male factor infertility and is used in nearly half of all IVF treatments. In ICSI a sperm is directly injected into each egg that is collected. The fertilized egg (embryo) is then transferred to your uterus.